Division of Chromatography

Heads of the Division : Zoltán Mezei, MD.
Assistant: Mária Csobán

This division was established in 1992 and since then it has undergone rapid development. One of its main tasks is the determination of the level of urine noradrenaline, adrenaline and dopamine, which are essential parameters in the diagnosis of catecholamine-secreting tumors (phaeochromocytoma, neuroblastoma, paraganglioma). We also measure the levels of urine non-conjugated catecholamine metabolite vanylmandalic acid with an average 1000 determination per year as well as serotonin metabolite 5-hydroxyindolacetic acid. From the year of 2008 the level of 11 antiepileptics (phenytoin, sultiame, zonisamide, 10-OH-carbazepine, desmethylmethsuximide, lamotrigine, ethosuximide, primidone, phenobarbital, carbamazepine-10,11-epoxide and carbamazepine) is measured by using HPLC. Since 2009, the levetiracetam (Keppra(R)) plasma concentration is also determined in this Division. Since 1996 HbA1C levels are analyzed in diabetic patients. Apart from HbA1C measurement liquid-chromatography system is assessed to detect other hemoglobin fractions as well. The instrument is also able to perform determinations directly from capillary blood, which are quite favorable in case of children and the elderly. This system has been upgraded and now it is possible to measure the level of HbF and HbA2 as important diagnostic parameters in the disease of β-thalassaemia. Helicobacter pylori infection is non-invasively investigated from breath sample by using isotope ratio mass spectrometry since 1998. Patients are usually examined after eradication therapy. The annual number of patients is about 20,000.