Division of Clinical Chemistry
Head of the Division : Anna V. Oláh, PhD
Chief Technologist: Edit Kalina
Clinical chemistry analyses have been performed since the foundation of our department (1978). At the beginning we were able to determine altogether 9 parameters on a Technicon SMA analyser. During the last few decades the list of available tests and the number of analysis have increased significantly. Currently, more then 64 parameters are determined, 37 of which are available on emergency call, too. Most routine tests are carried out on two COBAS Integra 800 and two Modular analysers. In addition, a Knauer and a VAPRO osmometer, a NOVA ionselective analyser and a spectrophotometer are also parts of the equipment. The present output of the division is 2-2.2 million tests per year. Most analyses are carried out in serum, but in some cases in plasma, urine, liquor and other body fluids as well. Tests can be requested individually or in panels. During the daily routine, results of the requested analyses are sent back to the clinicians via the Med-Solution on-line system.
The division also performs different types of electrophoretic assays of proteins, lipoproteins and enzymes for diagnostic purposes. The separation is made in agarose gel and the components are visualized by different staining methods. Quantitative analysis of components is performed with computerized densitometry. We perform annually 2500 manual analyses for the in- and outpatients of our university and for other health departments. The test menu of the division includes electrophoretic determination of serum proteins (albumin, α1-, α2-, β-, γ- globulins), LDH isoenzymes (LDH1, LDH2, LDH3, LDH4, LDH5, ratio of LDH1 and LDH2), lipoproteins as α(HDL), preβ(VLDL), β(LDL), chylomicron, CK isoenzymes (CK-MM, CK-MB, CK-BB), alkaline phosphatases (bone, liver isoforms, placental, intestinal isoenzymes) and immunofixation to detect monoclonal heavy chains (IgG, IgA, IgM) and monoclonal light chains (kappa, lambda). The indications of electrophoretic test are: myeloma multiplex, hemolysis, lipoprotein metabolism disorders, muscle diseases, bone and liver diseases, and monoclonal gammopathies. Since 2007 we investigate the distribution and clonality of IgG in cerebrospinal fluid and its corresponding serum by electrophoresis and isoelectric focusing, while local synthesis of IgG is a diagnostic marker of multiple sclerosis.