Division of Flow Cytometry

Head of Division : Zsuzsanna Hevessy, MD, PhD

Chief technologist: Valéria Kiss-Sziráki

The Division of Flow Cytometry in the Department of Laboratory Medicine was established in 1995 with the primary objective of assisting the diagnosis of acute and chronic leukemias. In the beginning, 3-4 samples were investigated weekly using only surface CD markers. A 2-color analysis was initially performed using the Becton Dickinson FACScan flow cytometer. In the past 10 years the sample number has risen to over 1500 per year. In our current routine, 4-color staining on a FACSCalibur flow cytometer is used and appropriate panels containing surface, cytoplasmic and nuclear antigens are available for each patient based on the initial diagnosis. In addition to leukemia immunophenotyping some prognostic factors are also investigated such as Zap-70 expression in CLL patients, DNA ploidy of the leukemic cells (DNA index and cell cycle) and multidrug resistance detected by a functional test. Flow cytometry is also an excellent tool in the analysis of rare receptor deficiencies like Glanzmann thrombasthenia and paroxysmal nocturnal hemoglobinuria (PNH). P-selectin analysis is applied to detect platelet hyperactivity. We provide CD34-positive progenitor cell enumeration - that is essential for stem cell transplantation - in a 24-hour service. The result of leukocyte immunophenotyping contains the percentage positivity of the CD markers and a short comment on the case including the most likely diagnosis. Findings are forwarded online via MedSolution program to the departments of the Medical and Health Science Center, while results are sent by fax or e-mail to other hematology centers in North-Eastern Hungary.